Location of the Ichetucknee River The Ichetucknee River and Springs are located within the confines of the Ichetucknee Springs State Park, which occupies 2,241 acres in Columbia and Suwannee counties and was established on January 6, 1970. The park's namesake is a series of freshwater springs that feed 233 million gallons of water daily into the sparkling Ichetucknee River. This river flows for six miles through hammock and swamp before joining the Santa Fe River. Driving Directions From Jacksonville... Take 10 west to US 90 exit,  follow 90 to Lake City to 41 south,  follow 41 south to SR 47 south,  follow park signs. From Tallahassee... Take US 27 south and follow signs.  or take I-10 east to I-75 south to exit #81,  take SR 47 south, follow park signs. From Gainesville... Take US 441 north to High Springs,  US 27 north to Fort White and follow signs.  or take I-75 north to exit #78,  take US 441 north to High Springs,  US 27 north to Fort White, and follow signs.